细胞代谢与微环境，DNA损伤修复及肿瘤治疗

主持科研项目

1.国家自然科学基金青年项目，RNF8经调控galectin-3泛素化修饰影响黑色素瘤免疫微环境的机制研究，2023.1-2025.12，主持，在研

2.兰州市青年科技人才创新项目，肿瘤微环境重塑对肿瘤转移影响的研究， 2023.11-2025.10，主持，在研

3.36365线路检测中心中央高校定向探索项目，RNF8经GAPDH介导瘤内成纤维细胞代谢重编程影响黑色素瘤转移机制的研究，2022.7-2024.6，主持，在研

4.横向项目，RNF8在ACSL4介导的肝脏脂代谢中的作用及调控机制研究， 2022.4-2024.4，主持，在研

5.科技部重点研发项目，裸鼹鼠、长爪沙鼠等五种特色实验动物的辐射损伤、代谢性紊乱、抗血吸虫病、帕金森病、脑缺血等疾病模型的创建及关键技术研究， 2022.1-2026.11，参与，在研

6.国家自然科学基金面上项目，泛素连接酶RNF8通过调控UQCRC2影响精子活力的机制研究， 2021.1-2024.12，参与，在研

7.国家自然科学基金面上项目，RAD6B通过组蛋白翻译后修饰调节Snail1转录及结肠癌转移机制的研究， 2021.1-2024.12，参与，在研

8.甘肃省普通中医药科研课题，当归补血汤抗放射性心肌损伤的物质基础及作用机制研究， 2023.1-2026.12，参与，在研

9.36365线路检测中心科技创新平台建设项目，基于微环境的肿瘤免疫治疗研究， 2022.11-2024.12，参与，在研

10.甘肃省自然科学基金，结合拉曼光谱技术研究RNF8基因对脂代谢的调控机制， 2021.11-2023.11，主持，结项

11.36365线路检测中心中央高校定向探索项目，共焦拉曼光谱仪在检测重金属镉所致肾损伤中的应用， 2018.1-2019.12，主持，结项

代表性论文

1. Guo Z, Song HY, Tian YX, Xu J, Zhang GK, Guo YA, Shen R^*, Wang DG^*. siRNF8 Delivered by DNA Framework Nucleic Acid Effectively Sensitizes Chemotherapy in Colon Cancer. INT J NANOMED. 2024;19:171-188.

2. Hu X, Yuan X, Zhang G, Song H, Ji P, Guo Y, Liu Z, Tian Y, Shen R^*, Wang DG^*. The intestinal epithelial-macrophage-crypt stem cell axis plays a crucial role in regulating and maintaining intestinal homeostasis. Life Sci. 2024:122452.

3. Liu DS, Wang L, Ha WH, Li K, Shen R^*, Wang DG^*. HIF-1alpha: A potential therapeutic opportunity in renal fibrosis. Chem Biol Interact. 2024;387:110808.

4. Zhang GK, Ji PF, Xia P, Song HY, Guo Z, Hu XH, Guo YN, Yuan XY, Song YF, Shen R^*, Wang DG^*. Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma. Comput Biol Med. 2023;167.

5. Guo YN^#, Shen R^#, Yang KR, Wang YT, Song HY, Liu XW, Cheng X, Wu RL, Song YF, Wang DG. RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma. Cell Death Discov. 2023;9(1).

6. Guo YN^#, Shen R^#, Wang F, Wang YT, Xia P, Wu RL, Liu XW, Ye WC, Tian YX, Wang DG. Carbon ion irradiation induces DNA damage in melanoma and optimizes the tumor microenvironment based on the cGAS-STING pathway. J Cancer Res Clin. 2023;149(9):6315-6328.

7. Ji PF, Zhang GK, Guo YN, Song HY, Yuan XY, Hu XH, Guo Z, Xia P, Shen R^*, Wang DG^*. Protein crotonylation: An emerging regulator in DNA damage response. Life Sci. 2023;331.

8. Xia P, Ouyang SW, Shen R, Guo Z, Zhang GK, Liu XW, Yang XG, Xie K, Wang DG. Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis. Int J Mol Sci. 2023;24(10).

9. Song HY^#, Shen R^#, Liu XW, Yang XG, Xie K, Guo Z, Wang DG. Histone post-translational modification and the DNA damage response. Genes Dis. 2023;10(4):1429-1444.

10. Song HY, Shen R, Mahasin H, Guo YN, Wang DG. DNA replication: Mechanisms and therapeutic interventions for diseases. Medcomm. 2023;4(1).

11. Shen R, Yang KR, Cheng X, Guo CL, Xing XQ, Sun HN, Liu DS, Liu XW, Wang DG. Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway. Environ Pollut. 2022;300.

12. Shen R, Liu DS, Wang XN, Guo Z, Sun HA, Song YF, Wang DG. DNA Damage and Activation of cGAS/STING Pathway Induce Tumor Microenvironment Remodeling. Front Cell Dev Biol. 2022;9.

13. Wu DM^#, Li J^#, Shen R^#, Li J, Yu Y, Li L, Deng SH, Liu T, Zhang T, Xu Y, Wang DG. Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury Inhibition of the NLRP3 Inflammasome. Front Pharmacol. 2022;12.

14. Ye Q, Wang JQ, Liu XW, Liu ZH, BaZong LS, Ma JH, Shen R^*, Ye WC^*, Zhang WF^*, Wang DG^*. The Role of RAD6B and PEDF in Retinal Degeneration. Neuroscience. 2022;480:19-31.

15. Yu LH^#, Shen R^#, Guo YN, Guo YX, Li C, Song YF, Wang DG. Loss of the ubiquitin-conjugating enzyme Rad6B disturbs mitochondrial function and cellular homeostasis in mouse skin. Biocell. 2021;45(3):761-772.

16. Li YE^#, Shen R^#, Wu HN, Yu LH, Wang Z, Wang DG. Liver changes induced by cadmium poisoning distinguished by confocal Raman imaging. Spectrochim Acta A. 2020;225.

17. Ma YP^#, Song YF^#, Shen R^#, Li PP, Ding H, Guo Z, Liu XW, Wang DG. Loss of RAD6B induces degeneration of the cochlea in mice. Biochem Bioph Res Co. 2020;531(3):402-408.

18. Guo YN^#, Shen R^#, Yu LH, Zheng X, Cui R, Song YF, Wang DG. Roles of galectin-3 in the tumor microenvironment and tumor metabolism. Oncol Rep. 2020;44(5):1799-1809.

19. Shen R, Li YE, Yu LH, Wu HN, Cui R, Liu S, Song YF, Wang DG. Ex vivo detection of cadmium-induced renal damage by using confocal Raman spectroscopy. J Biophotonics. 2019;12(12).

20. Shen R, Liu DS, Hou CC, Liu D, Zhao LX, Cheng J, Wang DG, Bai DC. Protective effect of polysaccharide on cadmium-induced nephrotoxicity and in vivo. Food Funct. 2017;8(10):3636-3646.

21. Shen R, Liu D, Hou CC, Cheng J, Bai DC. New fluorescent probe for Zn imaging in living cells and plants (vol 8, pg 83, 2016). Anal Methods-Uk. 2016;8(11):2541-2541.

22. Yang Y, Feng Y, Li H, Shen R, Wang YZ, Song XR, Cao C, Zhang GL, Liu WS. Hydro-soluble NIR fluorescent probe with multiple sites and multiple excitations for distinguishing visualization of endogenous Cys/Hcy, and GSH. Sensor Actuat B-Chem. 2021;333.

23. Sun CL, Li J, Wang XZ, Shen R, Liu S, Jiang JQ, Li T, Song QW, Liao Q, Fu HB, Yao JN, Zhang HL. Rational Design of Organic Probes for Turn-On Two-Photon Excited Fluorescence Imaging and Photodynamic Therapy. Chem-Us. 2019;5(3):600-616.

24. Wang YZ, Li BY, Song XR, Shen R, Wang DG, Yang Y, Feng Y, Cao C, Zhang GL, Liu WS. Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay. Anal Chem. 2019;91(19):12422-12427.

25. Huang H, Li TY, Sun YF, Yu LH, Wang CD, Shen R, Ye WC, Wang DG, Li YM. Amperometric sensing of hydrazine in environmental and biological samples by using CeO-encapsulated gold nanoparticles on reduced graphene oxide. Microchim Acta. 2019;186(1).

26. Yang Y, Shen R, Wang YZ, Qiu FZ, Feng Y, Tang XL, Bai DC, Zhang GL, Liu WS. A selective turn-on fluorescent sensor for Hg (II) in living cells and tissues. Sensor Actuat B-Chem. 2018;255:3479-3487.

27. Liu YM, Shen R, Ru JX, Yao X, Yang Y, Liu HL, Tang XL, Bai DC, Zhang GL, Liu WS. A reversible rhodamine 6G-based fluorescence turn-on probe for Fe in water and its application in living cell imaging. Rsc Adv. 2016;6(113):111754-111759.

28. Sun CL, Liao Q, Li T, Li J, Jiang JQ, Xu ZZ, Wang XD, Shen R, Bai DC, Wang Q, Zhang SX, Fu HB, Zhang HL. Rational design of small indolic squaraine dyes with large two-photon absorption cross section. Chem Sci. 2015;6(1):761-769.

29. Zhang KM, Dou W, Li PX, Shen R, Ru JX, Liu W, Cui YM, Chen CY, Liu WS, Bai DC. A coumarin-based two-photon probe for hydrogen peroxide. Biosens Bioelectron. 2015;64:542-546.

30. Li J, Sun CL, Shen R, Cao XY, Zhou B, Bai DC, Zhang HL. An Electrochemically Switched Smart Surface for Peptide Immobilization and Conformation Control. J Am Chem Soc. 2014;136(31):11050-11056.

31. Chen FJ, Liu GZ, Shi YJ, Xi PX, Cheng J, Hong JP, Shen R, Yao XJ, Bai DC, Zeng ZZ. A coumarin-derived fluorescent chemosensor for selectively detecting Cu: Synthesis, DFT calculations and cell imaging applications. Talanta. 2014;124:139-145.

教学研究论文

1.诸春敏,沈蓉,王德贵.浅谈提升“局部解剖学”教学质量的主要环节. 教育教学论坛. 2023(10).

2.王江昆,沈蓉,张朗,洪建平,杨旭光,王德贵.基于数字教育资源的局部解剖学智慧教学探索与实践，中国医学教育技术. 2023,37(05).

3.沈蓉,王江昆,刘向文,杨旭光,宋焱峰,王德贵.智慧教育在基础医学教育中的运用[J].甘肃教育,2023,724(08):92-96.

4.沈蓉, 王江昆, 王德贵,杨旭光,诸春敏.线上线下相结合教学模式在局部解剖学中的初探[J].医药卫生,2022(3):0183-0185

专利

一种检测锌离子的荧光分子探针及其制备方法,2015-8-19,中国, ZL20141021567.8。

一种蕨麻总黄酮提取设备，2020-6-19，中国，ZL201910338979.6。